Johnny Cash recorded a version of "God's Gonna Cut You Down" on American V: A Hundred Highways in 2003, with an arrangement quite different from most known gospel versions of the song. The song is presented with a rhythmic stomp-clap downbeat, grim and pessimistic in its portrayal of a vengeful and uncompromising God, serving as a warning to sinners.
A music video, directed by Tony Kaye, was made for this version in late 2006. It featured a number of celebrities, including Iggy Pop, Kanye West, Chris Martin, Kris Kristofferson, Patti Smith, Terrence Howard, Flea, Q-Tip, Adam Levine, Chris Rock, Justin Timberlake, Kate Moss, Sir Peter Blake, Sheryl Crow, Dennis Hopper, Woody Harrelson, Amy Lee, Scott Weiland, the Dixie Chicks, Mick Jones, Sharon Stone, Bono, Shelby Lynne, Anthony Kiedis, Travis Barker, Lisa Marie Presley, Kid Rock, Jay-Z, Keith Richards, Billy Gibbons, Corinne Bailey Rae, Johnny Depp, Graham Nash, Brian Wilson, Rick Rubin, Whoopi Goldberg, Owen Wilson and Jerry Lee Lewis. It also briefly features archive footage of Cash himself. The video was shot entirely in black and white. Since its release, both the song and video have seen moderate airplay.
The video won the 2008 Grammy Award for Best Short Form Music Video.